Gene Editing, BSL3, and Flow Cytometry
Gene Editing Core (GEE)
Gene Editing Core (GEE)
Genome Engineering
The Gene Editing Core (GEE) offers expert support and advanced technologies for generating knockout and knock-in models in cell lines and progenitor cells. With years of experience, the GEE core helps investigators achieve high-efficiency genome modifications in a time- and cost-effective manner.
Services:
- CRISPR/Cas9-based genome editing in mammalian cells
- Guide RNA design, reagent preparation, and transfection/infection
- Single-cell cloning, screening, and off-target evaluation
- Consultation and troubleshooting throughout the editing workflow
Biosafety Level 3 Core (BSL)
Biosafety Level 3 Facility (BSL)
High-Containment Research
The BSL3 Core provides a secure, specialized facility for research involving Risk Group 3 (RG3) infectious agents. Designed with advanced engineering controls and stringent operational protocols, the BSL3 Core minimizes risk to personnel, the environment, and the community.
Services:
- Secure space for handling RG3 pathogens under BSL-3 containment
- Support for infectious disease research in microbiology, virology, and immunology
- Biosafety oversight and protocol consultation for high-risk studies
Facility Features & Equipment:
- Four biosafety cabinets, six CO₂ incubators supporting six concurrent researchers
- Beckman Optima L-90K Ultracentrifuge: SW60Ti, SW-32, and SW41
Flow Cytometry Core (FLO)
Flow Cytometry Core (FLO)
The Flow Cytometry Core (FLO) provides advanced instrumentation and expert support for multi-parameter analysis of cells and particles using fluorescence-based flow cytometry. FLO enables high-sensitivity detection, rare event analysis, and cell sorting to support a wide range of basic and translational research applications.
Services:
- Multi-color immunophenotyping and rare cell analysis
- Nanoparticle detection and high-resolution fluorescence sensitivity
- Automated sampling using 96-well plate loaders
- Training, technical assistance, and consultation on panel design and data acquisition
Equipment:
- CytoFLEX S (Beckman Coulter): 4-laser, 13-parameter cytometer with APD-based detection; located in BSL-3 for infectious sample handling
- Amnis CellStream (Luminex): 4-laser, 17-parameter system with TDI CCD imaging for enhanced fluorescence and morphology detection
- FACSAria III (BD Biosciences): 3-laser, 9-color flow cytometer with cell sorting capabilities and ACDU for multi-well plate deposition
Contacts
For reservations and a free consultation, register on the Meharry iLab site iLab Organizer :: login.